Analysis of Genetic Variation in Mitochondrial DNA of Cyprinus carpio Using Restriction Enzymes
ABSTRACT
The study involved isolating mitochondrial DNA (mtDNA) from common carp (Cyprinus carpio) collected from regions near Pondicherry and Tamil Nadu to assess genetic variation. Restriction digestion analysis revealed variability in mtDNA sequences among fish from different locations, indicating molecular-level genetic divergence. These differences are likely influenced by environmental and geographical factors. The detection of distinct mtDNA haplotypes suggests that the carp populations may originate from separate gene pools shaped by varying agro-climatic conditions. These findings highlight the genetic diversity within regional carp populations and may be useful in selecting robust and adaptable fish stocks for sustainable aquaculture practices.
KEYWORDS: Mitochondrial DNA, Genetic variation, Environment, Carp species, Restriction digestion
